For my first mini-project I used DLS to analyse the kinetics of two different dynamic processes that increase or decrease in size over time. This data will be used in future to help build a new DLS data analysis program for dynamic processes that change size over time (i.e. clathrin cage formation and disassembly), of which should be more accurate compared to traditional methods of DLS analysis. The two model systems analysed include SMALP nanodisc formation and beta-lactoglobulin aggregation. Circular dichroism of beta-lactoglobulin at different temperatures was also conducted to determine changes in protein structure before and after aggregation.

For my second mini-project I am looking at obtaining a structure of clathrin-only cages, of which will aid structural biology investigation into clathrin and adaptor protein interactions. Additional experiments are also being conducted which look at adaoptor protein and clathrin interaction by using a GST pull down. Techniques I have learned so far during my mini-project include SDS-page and a GST pull down assay (affinity-chromatography). Techniques I will be learning in the near-future will include clathrin purification and cryo-electron microscopy.

For my undergraduate masters I completed a project in Dr Loverings lab at the University of Birmingham. My project focused on determining structural and biochemical characteristics of the gliding motility complex found in Bdellovibrio bacteriovorus. I learned several experimental techniques including PCR, affinity chromatography, size exclusion chromatography, bacteria culture preparation, SDS page, crystallography screens, and spectroscopy,

I was awarded £2500 funding from the BBSRC to conduct a research experience placement within the Hidalgo lab at the University of Birmingham for 8 weeks. My project focused on utilising Drosophila to determine the location of a gene which interacts with the transcription factor Prospero. During the project I developed new lab skills including genetic crossing, adult and L3 larvae brain dissections, survival assays, and adult and L3 larvae locomotion behavioural studies.